Howard Salis

IEE Faculty Member
Titles and Affiliations
Associate Professor, Agricultural & Biological Engineering
Associate Professor, Chemical Engineering

Research Summary

Focuses on the development of rational design methods for engineering synthetic biological systems - metabolic pathways, genetic circuits and genomes. He develops kinetic and thermodynamic models of genetic regulation and tests those predictions in industrially and medically useful microorganisms to gain a quantitative understanding of genetic function. He also programs synthetic microorganisms to manufacture useful chemicals for bioenergy applications and to better understand the dynamical behavior of natural microorganisms.

In the News

Research Keywords

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Recent Publications

Predicting synthetic mRNA stability using massively parallel kinetic measurements, biophysical modeling, and machine learning

Cetnar, D. P., Hossain, A., Vezeau, G. E. & Salis, H. M., Dec 2024, In: Nature communications. 15, 1, 9601.

Research output: Contribution to journalArticlepeer-review

An autonomous microbial sensor enables long-term detection of TNT explosive in natural soil

Essington, E. A., Vezeau, G. E., Cetnar, D. P., Grandinette, E., Bell, T. H. & Salis, H. M., Dec 2024, In: Nature communications. 15, 1, 10471.

Research output: Contribution to journalArticlepeer-review

Automated Design of Oligopools and Rapid Analysis of Massively Parallel Barcoded Measurements

Hossain, A., Cetnar, D. P., LaFleur, T. L., McLellan, J. R. & Salis, H. M., Dec 20 2024, In: ACS Synthetic Biology. 13, 12, p. 4218-4232 15 p.

Research output: Contribution to journalArticlepeer-review

Autoclaving is at least as effective as gamma irradiation for biotic clearing and intentional microbial recolonization of soil

King, W. L., Grandinette, E. M., Trase, O., Rolon, M. L., Salis, H. M., Wood, H. & Bell, T. H., Jul 2024, In: mSphere. 9, 7

Research output: Contribution to journalArticlepeer-review

Genetic circuitry boosts cell longevity

Salis, H. M., Apr 28 2023, In: Science. 380, 6643, p. 343 1 p.

Research output: Contribution to journalArticlepeer-review

Automated design of protein-binding riboswitches for sensing human biomarkers in a cell-free expression system

Vezeau, G. E., Gadila, L. R. & Salis, H. M., Dec 2023, In: Nature communications. 14, 1, 2416.

Research output: Contribution to journalArticlepeer-review

Automated model-predictive design of synthetic promoters to control transcriptional profiles in bacteria

LaFleur, T. L., Hossain, A. & Salis, H. M., Dec 2022, In: Nature communications. 13, 1, 5159.

Research output: Contribution to journalArticlepeer-review

Systematic Quantification of Sequence and Structural Determinants Controlling mRNA stability in Bacterial Operons

Cetnar, D. P. & Salis, H. M., Feb 19 2021, In: ACS Synthetic Biology. 10, 2, p. 318-332 15 p.

Research output: Contribution to journalArticlepeer-review

Selenium-dependent metabolic reprogramming during inflammation and resolution

Korwar, A. M., Hossain, A., Lee, T. J., Shay, A. E., Basrur, V., Conlon, K., Smith, P. B., Carlson, B. A., Salis, H. M., Patterson, A. D. & Prabhu, K. S., Jan 1 2021, In: Journal of Biological Chemistry. 296, 100410.

Research output: Contribution to journalArticlepeer-review

Purification of Cas9—RNA complexes by ultrafiltration

Manzano, I., Taylor, N., Csordas, M., Vezeau, G. E., Salis, H. M. & Zydney, A. L., Mar 1 2021, In: Biotechnology progress. 37, 2, e3104.

Research output: Contribution to journalArticlepeer-review

Tuning Cell-Free Composition Controls the Time Delay, Dynamics, and Productivity of TX-TL Expression

Vezeau, G. E. & Salis, H. M., Oct 15 2021, In: ACS Synthetic Biology. 10, 10, p. 2508-2519 12 p.

Research output: Contribution to journalArticlepeer-review

Synthesis Success Calculator: Predicting the Rapid Synthesis of DNA Fragments with Machine Learning

Halper, S. M., Hossain, A. & Salis, H. M., Jul 17 2020, In: ACS Synthetic Biology. 9, 7, p. 1563-1571 9 p.

Research output: Contribution to journalArticlepeer-review

Automated design of thousands of nonrepetitive parts for engineering stable genetic systems

Hossain, A., Lopez, E., Halper, S. M., Cetnar, D. P., Reis, A. C., Strickland, D., Klavins, E. & Salis, H. M., Dec 2020, In: Nature Biotechnology. 38, 12, p. 1466-1475 10 p.

Research output: Contribution to journalArticlepeer-review

Convolutional neural net learns promoter sequence features driving transcription strength

Leiby, N., Hossain, A. & Salis, H. M., Mar 11 2020, In: EPiC Series in Computing. 70, p. 163-172 10 p.

Research output: Contribution to journalConference articlepeer-review

RNA size and 3-dimensional structure determine ultrafiltration behavior of small RNA molecules

Manzano, I., Vezeau, G., Salis, H. & Zydney, A. L., Apr 15 2020, In: Separation and Purification Technology. 237, 116372.

Research output: Contribution to journalArticlepeer-review